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  • Role of PCNA and RFC in promoting Mus81-complex activity

    actively loaded onto DNA. This process requires a clamp loader, replication factor C (RFC), which loads PCNA onto primer-template junctions in an ATP-dependent manner [29, 30]. RFC is a heteropentameric complex composed of a large Rfc1 subunit (94.9 kDa) and four small subunits – Rfc2 (39.7 kDa), Rfc3 (38.2 kDa), Rfc4 (36 kDa), and Rfc5 (40 kDa).

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  • (PDF) Mechanism of PCNA Loading by RFC

    The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP binding to this subunit initiates RFC activation, and the clamp loader adopts a spiral conformation that

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  • Tracking Sliding Clamp Opening and Closing during

    Feb 25, 2000 · A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA. Journal of Molecular Biology 2012, 416 (2), 163-175. DOI: 10.1016/j.jmb.2011.12.017. Miho Sakato, Yayan Zhou, Manju M. Hingorani.

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  • DeepDyve - Unlimited Access to Peer-Reviewed Journals

    DeepDyve is the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

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  • PCNA Loading by RFC, Mechanism of | SpringerLink

    Up to12%cash back · Jan 30, 2018 · Sakato M, O'Donnell M, Hingorani MM (2012a) A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol 416(2):163–175 PubMed CrossRef Google Scholar Sakato M, Zhou Y, Hingorani MM (2012b) ATP binding and hydrolysis-driven rate-determining events in the RFC-catalyzed PCNA clamp loading reaction.

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  • 10.1016/S0022-2836(12)00044-7 | DeepDyve

    Jun 11, 2020 · Abstract. Volume 416, Issue 2 Contents Articles A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA ATP Binding and Hydrolysis-Driven RateDetermining Events in the RFC-Catalyzed PCNA Clamp Loading Reaction Energy Complexes Are Apparently Associated with the Switchâ Motor Complex of Bacterial Flagella Molecular Structure and …

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  • Michael E. O'Donnell - Publications

    A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. Journal of Molecular Biology. 416: 163-75. PMID 22197374 DOI: 10.1016/j.jmb.2011.12.017 : 1: 2012: Thompson JA, Marzahn MR, O'Donnell M, Bloom LB. Replication factor C is a more effective proliferating cell nuclear antigen (PCNA) opener than the checkpoint

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  • Journal of Molecular Biology | Vol 416, Issue 2, Pages 163

    Research article Full text access A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA

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  • Mapping the interaction of DNA with the Escherichia coli

    Jan 16, 2005 · The RFC–PCNA complex suggested to us a mechanism for the recognition of target DNA by the ATP-loaded clamp loader 19. In the RFC–PCNA …

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  • Alternative clamp loaders/unloaders | FEMS Yeast Research

    PCNA is a protein that forms a ring that encircles the replicating DNA. It plays major roles in DNA replication and repair, and serves as a signaling hub. Four different protein complexes act as clamp loaders/unloaders in the yeast Saccharomyces cerevisiae. These are conserved throughout the evolutionary scale.

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  • Exploration of Prognostic Biomarkers among Replication

    Feb 12, 2021 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol. 2012 ;416: 163 - 175 . Google Scholar | Crossref | Medline

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  • DeepDyve - Unlimited Access to Peer-Reviewed Journals

    DeepDyve is the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

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  • Structure of the human clamp loader reveals an

    Sep 09, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a reaction intermediate …

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  • Interactional similarities and differences in the protein

    Jun 14, 2019 · Sakato M, O'Donnell M, Hingorani MM. A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol. 2012;416:163–75. CAS Article Google Scholar 14. Cullmann G, Fien K, Kobayashi R, Stillman B. Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:4661

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  • Linchpin DNA-binding residues serve as go/no-go …

    A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA.

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  • Publications – Manju Hingorani

    Sakato, M., O'Donnell, M., Hingorani, M.M. (2012) "A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA" Journal of Molecular Biology 416, 163-75. Pieniazek, S.N., Hingorani, M.M., Beveridge, D.L. (2011) "Dynamical allosterism in the mechanism of action of DNA mismatch repair protein MutS" Biophysics

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  • Multifaceted regulation and functions of replication

    Aug 01, 2018 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol. 2012; 416 :163–175. [ PMC free article ] [ PubMed ] [ Google Scholar ]

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  • Journal of Molecular Biology | Vol 416, Issue 2, Pages 163

    Research article Full text access A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA

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  • (PDF) A structure-function analysis of the yeast Elg1

    The sliding clamp, PCNA, plays a central role in DNA replication and repair. In the moving replication fork, PCNA is present at the leading strand and at each of the Okazaki fragments that are formed on the lagging strand. PCNA enhances the

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  • Linchpin DNA-binding residues serve as go/no-go controls

    Moreover, their role depends on the clamp loader subunit; thus, Arg-84, Arg-88, and Arg-101 in RFC-B, RFC-C, and RFC-D, respectively, are increasingly important for stabilization of open PCNA upon ATP binding to RFC; Arg-88 in RFC-C is important for efficient positioning of ptDNA in the complex, which in turn enables further PCNA opening, ATP

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