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  • Interplay of clamp loader subunits in opening the beta

    DNA Polymerase Clamp Loaders. Chromosomal DNA replication requires that DNA polymerases be tethered to ring-shaped sliding clamps that encircle the DNA and allow for high-speed, processive replication. Sliding clamps are loaded onto DNA by the clamp loader complex, a pentameric assembly of proteins of the AAA+ family of ATPases.

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  • [PDF] Ordered ATP Hydrolysis in the γ Complex Clamp Loader

    Apr 18, 2003 · The γ complex couples ATP hydrolysis to the loading of β sliding clamps onto DNA for processive replication. The γ complex structure shows that the clamp loader subunits are arranged as a circular heteropentamer. The three γ motor subunits bind ATP, the δ wrench opens the β ring, and the δ′ stator modulates the δ-β interaction.

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  • DNA Polymerase Clamp Loaders - UMass Med

    Fig. 1. Clamp loaders and sliding clamps. ( A) Clamp-loading reaction. The clamp loader has low affinity for both clamp and primer-template DNA in the absence of ATP. Upon binding ATP, the clamp loader can bind the clamp and open it. The binding of primer-t emplate DNA activates ATP hydrolysis, leading to ejection of the clamp loader.

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  • Cryo-EM structures reveal high-resolution mechanism of a

    Sep 23, 2021 · 31 shaped sliding clamp proteins that wrap around DNA to activate 32 DNA polymerases and other factors (Moldovan et al., 2007). 33 Sliding clamps are regulated by their presence on DNA, which in 34 turn is governed by clamp loaders that open the sliding clamp ring 35 and place it onto DNA (Kelch, 2016). The clamp loader of

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  • DNA Replication: How Does a Sliding Clamp Slide

    The clamp loader traps a spiral conformation of the open clamp so that both the loader and the clamp match the helical symmetry of DNA. One structure reveals that ATP has been hydrolyzed in one subunit, and suggests that clamp closure and ejection of the loader involves disruption of the ATP-dependent match in symmetry.

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  • How a DNA polymerase clamp loader opens a sliding …

    The clamp loader traps a spiral conformation of the open clamp so that both the loader and the clamp match the helical symmetry of DNA. One structure reveals that ATP has been hydrolyzed in one subunit and suggests that clamp closure and ejection of the loader involves disruption of the ATP-dependent match in symmetry.

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  • Allosteric communication in DNA polymerase clamp loaders

    Apr 13, 2021 · Sliding DNA clamps and the ATP-driven clamp-loader complexes that load them onto DNA are essential components of the DNA replication machinery in all branches of life (Yao and O'Donnell, 2016).Sliding clamps, such as proliferating cell nuclear antigen (PCNA) in eukaryotes, are ring-shaped proteins that enable highly processive DNA replication by tethering DNA polymerases to the template

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  • Dynamics of Open DNA Sliding Clamps

    May 05, 2016 · Kelch BA, Makino DL, O'Donnell M, Kuriyan J. How a DNA polymerase clamp loader opens a sliding clamp. Science. 2011;334(6063):1675–80. Epub 2011/12/24. PubMed Central PMCID: PMC3281585. pmid:22194570 . View Article PubMed/NCBI Google Scholar 21. Fang J, …

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  • Allosteric communication in DNA polymerase clamp loaders

    Apr 13, 2021 · Sliding DNA clamps and the ATP-driven clamp-loader complexes that load them onto DNA are essential components of the DNA replication machinery in all branches of life (Yao and O'Donnell, 2016).Sliding clamps, such as proliferating cell nuclear antigen (PCNA) in eukaryotes, are ring-shaped proteins that enable highly processive DNA replication by tethering DNA polymerases to the template

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  • DNA clamp - enacademic.com

    Apr 20, 2012 · The clamp loader opens sliding clamps and places them on the DNA at the site of the primer-template junction in the correct orientation for polymerase to bind, both at the initiation of DNA synthesis on the leading strand and continually at the start of …

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  • Structural analysis of a eukaryotic sliding DNA clamp

    Jun 17, 2004 · Jeruzalmi, D. et al. Mechanism of processivity clamp opening by the δ subunit wrench of the clamp loader complex of E. coli DNA polymerase III. Cell 106, …

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  • Dynamics of Open DNA Sliding Clamps

    May 05, 2016 · Kelch BA, Makino DL, O'Donnell M, Kuriyan J. How a DNA polymerase clamp loader opens a sliding clamp. Science. 2011;334(6063):1675–80. Epub 2011/12/24. PubMed Central PMCID: PMC3281585. pmid:22194570 . View Article PubMed/NCBI Google Scholar 21. Fang J, …

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  • The RFC Clamp Loader: Structure and Function

    Scheme of clamp loader function. (a) The structures of the E. coli β and human PCNA sliding clamps (PDB: 2POL and PDB: AXC, respectively).(b) Schematic of clamp loader function using eukaryotic RFC and PCNA as the example.ATP binding to RFC enables RFC to bind and open PCNA. In the presence of a primed template, RFC places PCNA onto DNA and then hydrolyzes ATP to eject from the PCNA-DNA

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  • DNA Polymerase Clamp Loaders - UMass Med

    DNA Polymerase Clamp Loaders. Chromosomal DNA replication requires that DNA polymerases be tethered to ring-shaped sliding clamps that encircle the DNA and allow for high-speed, processive replication. Sliding clamps are loaded onto DNA by the clamp loader complex, a pentameric assembly of proteins of the AAA+ family of ATPases.

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  • The β Sliding Clamp Closes around DNA prior to Release by

    Escherichia coli γ complex clamp loader functions to load the β sliding clamp onto sites of DNA replication and repair. The clamp loader uses the energy of ATP binding and hydrolysis to drive conformational changes allowing for β binding and opening, DNA binding, and then release of the β·DNA complex. Although much work has been done studying the sliding clamp and clamp loader …

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  • How a DNA polymerase clamp loader opens a sliding clamp

    Dec 01, 2011 · Instead, ATP-dependent complexes known as clamp loaders open the sliding clamps and load them on to primed DNA in the correct orientation for productive engagement of the polymerase (the clamp loaders are the γ/τ complex in bacteria, Replication Factor-C (RFC) in eukaryotes and archaea, and gp44/62 in T4 bacteriophage (Fig. 1B)).

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  • pcn - DNA polymerase sliding clamp - Methanosarcina sp

    Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.

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  • DNA polymerase by throwing in dideoxy ATP in tube A

    o Main part of the polymerase that is active is the core, sliding clamp makes sure we stay at the active site of the 3' end we are elongating, because okazaki fragments and we keep making a new primer we use the sliding clamp loader which uses ATP to open the beta subunit Bringing lagging strand to the core of the polymerase Another view of

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  • pcn - DNA polymerase sliding clamp - Methanosarcina sp

    Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.

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  • DNA Replication: How Does a Sliding Clamp Slide

    Mar 06, 2017 · The other core component is the clamp loader, a pentameric complex that uses ATP to open and close the clamp around DNA, and is ubiquitous in all cells 3, 6. Sliding clamps are now known to be used in many processes beyond replication, including repair, cell cycle control, and nucleosome assembly . Because of the unusual nature of the clamp

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